In a recent project, we identified NOS2, the gene encoding iNOS, as a putative target gene of the histone methyltransferase enhancer of zeste homolog 2 (Ezh2) in … In macrophages, NO mediates tumoricidal and bactericidal actions. anti-NOS2 Antibody reacting with Rabbit and identified with WB, IHC, IF, ELISA. Immunofluorescence staining of NOS2 in Hela cells. NOS2 helps neurotransmission and has antimicrobial and antitumor activities ( R ). a free radical. Anti-NOS2 antibodies are offered by a number of suppliers. We are pleased to announce some changes to the ongoing curation of the data in SFARI Gene. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2. NOS2 (Nitric Oxide Synthase 2, Inducible, NOS2) Short Description: Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. HEP-NOS, iNOS, NOS, NOS2A. This antibody recognizes human,mouse antigen. The enzyme nitric oxide synthase 2 (NOS2), often called inducible NOS, plays a central role in the inflammatory reactions that follow infection or tissue damage. ACKNOWLEDGMENTS. NOS2 Antibody (C-11) is a high quality monoclonal NOS2 antibody (also designated iNOS antibody or NOS2A antibody) suitable for the detection of … As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S … The human NOS2 promoter contains multiple potential binding sites for transcription factors including binding sites for NFjB, activator protein 1 (AP-1), CCAAT/enhancer-binding protein (C/EBP β) … Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human NOS2 polyclonal antibody (dilution ratio 1:1000) at 4℃ overnight. NOS2: Description: nitric oxide synthase 2 [Source:HGNC Symbol;Acc:HGNC:7873] Organism: Homo sapiens: Synonym(s) 163728, a1l3u5, b7zly2, hep-nos, inos, … View mouse Nos2 Chr11:78920787-78960226 with: phenotypes, sequences, polymorphisms, proteins, references, function, expression It helps in immune defense against pathogens by producing nitric oxide. Cloning of NOS enzymes indicates that cNOS include both brain constitutive ( NOS1) and endothelial constitutive ( NOS3 ); the third is the inducible ( NOS2) gene. Genetic association has been found between the NOS2 gene (formerly known as the NOS2A gene) and autism in a Korean population cohort (Kim et al., 2009). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). iNOS Inducible nitric oxide synthase (iNOS), along with neuronal nitric oxide synthase (nNOS) and endothelial nitric oxide synthase (eNOS), catalyze the generation of nitric oxide and L-citrulline from L-arginine and molecular oxygen. They lack the gene encoding nitric oxide synthase 2 (Nos2) and are susceptible to murine CMV infection. The OD value is proportional to the concentration of Human NOS2/iNOS. Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Homo sapiens (human) : NOS2 (nitric oxide synthase 2) HGNC Alliance Mus musculus (house mouse) : Nos2 (nitric oxide synthase 2, inducible) MGI Alliance Chinchilla lanigera (long-tailed chinchilla) : Nos2 (nitric oxide synthase 2) Pan paniscus (bonobo/pygmy chimpanzee) : NOS2 (nitric oxide synthase 2) The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. iNOS in Human Brain. It displays high affinity for Ca 2+ /calmodulin. Whereas the function of Nos2 in murine host immunity is well characterized, that of human NOS2 has remained elusive and controversial. quantity: 150UL price: 299 USD to the supplier NANOS2 (Nanos C2HC-Type Zinc Finger 2) is a Protein Coding gene. Molecular Function This gene encodes a nitric oxide synthase which is inducible by a combination o f lipopolysaccharide and certain cytokines. Activated mouse macrophages metabolize arginine via NO synthase (NOS2) to produce NO as an antimicrobial effector. Human(4843) Summary: Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. NOS2 Uniprot Gene Name NITRIC OXIDE SYNTHASE, INDUCIBLE Uniprot Protein Name ENSG00000007171 Ensembl Gene Id Structurally, the protein is reported to be 131.1 kilodaltons in mass. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. NOS2 gene product. The results showed that P. gingivalis coated with anti -P. gingivalis antibody before co-culture with neuron cells could decrease the gene expression of TNF , IL1B , and NOS2 of neuron cells. Nitric oxide synthase-2 (NOS2) is a key gene that displays species-specific outcomes via altered regulation of the gene promoter and via post-transcriptional mechanisms in humans that are not found in mice. Generation of NO requires the coordinated regulation of multiple genes. Quality is guaranteed. The concentration of Human NOS2/iNOS in samples can be calculated by comparing the OD of the samples to the standard curve. Autosomal recessive NOS2 deficiency has been described in mice. Homologs of the NOS2 gene: The NOS2 gene is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, and frog. This target gene encodes the protein 'nitric oxide synthase 2' in humans and may also be known as nitric oxide synthase 2, inducible, HEP-NOS, INOS, NOS, NOS2A, and nitric oxide synthase, inducible. This model demonstrates an altered immune redox state reminiscent of the human disease and capitalizes on data indicating critical differences between human and mouse immune responses, particularly in nitric oxide levels produced by immune activation of the NOS2 gene. Because NOS2-mediated NO production is primarily regulated at the transcriptional level, studies of genetic determinants of NO expression in malaria have focused on the 5′ promoter region of the NOS2 gene. This work was supported by the Wellcome Trust and The Medical Research Council. qSTAR qPCR primer pairs against Homo sapiens gene NOS2. Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. (from RefSeq NM_000625) RefSeq Summary (NM_000625): Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Diseases associated with NANOS2 include Spermatocytoma and Spermatogenic Failure, X-Linked, 1. NOS2 encodes Inducible NOS (iNOS) in the immune system and cardiovascular system. In contrast, mouse macrophages contain unmethylated cytosine-phosphate-guanine (CpG) dinucleotides proximal to the NOS2 transcription start site. The human NOS2 gene is highly methylated around the NOS2 transcription start site. First Strand cDNA Synthesis Kit (11801-025) NOS2 is involved in inflammatory responses and enhances the synthesis of PGE2 and proinflammatory cytokines such as IL-6 and IL-8. SFARI Gene Update. Recently, NOS activity has been demonstrated in several bacterial species, including notorious pathogens Bacillus anthracis and Staphylococcus aureus. Johannesen et al. The NOS2 antibody from Proteintech is a rabbit polyclonal antibody to a fusion protein of human NOS2. Published gene expression datasets provide little support for the activation of this pathway in human macrophages. NOS2 has been detected in virtually every cell type, and the NO it produces can perform both beneficial and detrimental actions. iNOS was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Mouse Anti-Human/Mouse/Rat iNOS Monoclonal Antibody (Catalog # MAB9502) at 5 µg/mL overnight at 4 °C. The NOS2 antibody has been validated for the following applications: IHC, WB, ELISA analysis. The resulting levels of NO produced by activation of human NOS2 are different from the levels of NO produced by mouse Nos2. The gene expressions of the TNF , IL1B , NOS2 in neurons was evaluated using RT-qPCR. Transmission disequilibrium testing was performed using 257 Danish IDDM families; 154 families were affected sib pair families, and 103 families were simplex families. An important paralog of this gene is NANOS3. Gene name: NOS2 (HGNC Symbol) Synonyms: HEP-NOS, iNOS, NOS, NOS2A: Description: Nitric oxide synthase 2 (HGNC Symbol) Chromosome: 17: Cytoband: q11.2: Chromosome location (bp) 27756766 - 27800499: Number of transcripts i In the context of a continued effort to develop the human gene module and its manually curated list of autism risk genes, we are modifying other aspects of the site to focus on the information that is of greatest interest to the research community. Product Pages: Species: Gene Names: Gene Aliases: RefSeq Accessions: SNP IDs (if applicable): Mature Names (if applicable): Hs00164883_CE details, Hs00164883_CE search: Human Orthologs from Annotation Pipeline: 271 organisms have orthologs with human gene NOS2; Orthologs NP100041. Add To Cart. We have generated RNA-sequencing data from bone marrow–derived macrophages … Gene Ontology (GO) annotations related to this gene include mRNA binding. The HL-60 hca cell line provides a tool for exploring these issues and the general question of how the human NOS2 gene is regulated. Contact Us | Register or Login ... NOS2 (Myc-DDK-tagged)-Human nitric oxide synthase 2, inducible (NOS2) 10 ug; USD 1,210.00. (2001) scanned all 27 exons of the human NOS2 gene and carried out linkage transmission disequilibrium testing of the identified NOS2 polymorphisms in a Danish nationwide IDDM family collection. 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